Ministry of Environment and Forests NotificationNew Delhi, the 5th December, 1989
Rules for the Manufacture, use, import, export and storage of hazardous micro organisms, genetically engineered organisms or cells
(To be notified under the EP Act, 1986)
G.S.R. 1037(E) – In exercise of the powers conferred by sections 6, 8 and 25 of the Environment (Protection) Act, 1986 (29 of 1986) and with a view to protecting the environment, nature and health, in connection with the application of genetechnology and micro-organisms, the Central Government hereby makes the following rules, namely:-
1. Short title, extent and commencement. – (1) These rules may be called the Rules for The Manufacture, Use, Import, Export and Storage of Hazardous micro-organisms Genetically engineered organisms or cells.
(2) These rules shall come into operation on the date to be notified for this purpose in the Official Gazette.
2. Application – (1) These rules are applicable to the manufacture import and storage of micro-organisms and Gene-Technological products.
(2) These shall apply to genetically engineered organisms, micro-organisms and cells and correspondingly to any substances and products and food stuffs, etc. of which such cells, organisms or tissues hereof form part.
(3) These rules shall also apply to new genetechnologies apart from those referred to in clauses (ii) and (iv) of rule 3 and these rules shall apply to organisms/micro-organisms and cells generated by the utilization of such other gene-technologies and to substances and products of which such organisms and cells form part.
(4) These rules shall be applicable in the following specific cases:
(a)sale, offers for sale, storage for the purpose of sales, offers and any kind of handling over with or without a consideration;
(b)exportation and importation of genetically engineered cells or organisms;
(c)production, manufacturing, processing, storage, import, drawing off, packaging and repacking of the Genetically Engineered Products;
(d)Production, manufacture etc., of drugs and pharmaceuticals and food stuffs distilleries and tanneries, etc. which make use of micro-organisms genetically engineered micro-organisms one way or the other.
These rules shall be applicable to the whole of India.
3. Definitions. – In these rules unless the context requires,
“Biotechnology” means the application of scientific and engineering principles to the processing of materials by biological agents to produce goods and services;
(ii) “Cell hybridisation” means the formation of live cells with new combinations of genetic material through the fusion of two or more cells by means of methods which do not occur naturally;
(iii) “Gene Technology” means the application of the gene technique called genetic engineering, include self cloning and deletion as well as cell hybridisation;
(iv) “Genetic engineering” means the technique by which heritable material, which does not usually occur or will not occur naturally in the organism or cell concerned, generated outside the organism or the cell is inserted into said cell or organism. It shall also mean the formation of new combinations of genetic material by incorporation of a cell into a host cell, where they occur naturally (self cloning) as well as modification of an organism or in a cell by deletion and removal of parts of the heritable material;
“microorganisms” shall include all the bacteria, viruses, fungi, mycoplasma, cells lines, algae, protodones and nematotes indicated in the schedule and those that have not been presently known to exist in the country or not have been discovered so far”.
4. Competent Authorities. – (1) Recombinant DNA Advisory Committee (RDAC)
This committee shall review developments in Biotechnology at national and international levels and shall recommend suitable and appropriate safety regulations for India in recombinant research, use and applications from time to time. The committee shall function in the Department of Biotechnology.
(2) Review Committee on Genetic Manipulation (RCGM).
This committee shall function in the Department of Biotechnology to monitor the safety related aspect in respect of on-going research projects and activities involving genetically engineered organisms/hazardous microorganisms. The Review Committee on Genetic Manipulation shall include representatives of (a) Department of Biotechnology (b) Indian Council of Medical Research (c) Indian Council of Agricultural Research (d) Council of Scientific and Industrial Research (e) other experts in their individual capacity. Review Committee on Genetic Manipulation may appoint sub groups.
It shall bring out Manuals of guidelines specifying procedure for regulatory process with respect to activities involving genetically engineered organisms in research, use and applications including industry with a view to ensure environmental safety. All ongoing projects involving high risk category and controlled field experiments shall be reviewed to ensure that adequate precautions and containment conditions are followed as per the guidelines.
The Review Committee on Genetic Manipulation shall lay down procedures restricting or prohibiting production, sale, importation and use of such genetically engineered organisms of cells as are mentioned in the Schedule.
(3) Institutional Biosafety Committee (IBSC).
This committee shall be constituted by an occupier or any person including, research institutions handling micro organisms/genetically engineered organisms. The committee shall comprise the Head of the Institution, Scientists engaged in DNA work, a medical expert and a nominee of the Department of Biotechnology. The occupier or any person including research institutions handling microorganisms/genetically engineered organisms shall prepare with the assistance of the Institutional Biosafety Committee (IBSC) an up-to-date on-site emergency plan according to the manuals/guidelines of the RCGM and make available copies to the District Level Committee/State Biotechnology Co-ordinating Committee and the Genetic Engineering Approval Committee.
(4) Genetic Engineering Approval Committee (GEAC)
This committee shall function as a body under the Department of Environment, Forests and Wildlife for approval of activities involving large scale use of hazardous microorganisms and recombinants in research and industrial production from the environmental angle. The Committee shall also be responsible for approval of proposals relating to release of genetically engineered organisms and products into the environment including experimental field trials.
The composition of the Committee shall be
(i) Chairman – Additional Secretary, Department of Environment, Forests and Wild life.
Co-Chairman – Representative of Department of Bio-technology
(ii) Members: Representatives of concerned Agencies and Departments, namely, Ministry of Industrial Development, Department of Biotechnology and the Department of Atomic Energy.
(iii) Expert members: Director General – Indian Council Research, Director General – Indian Council of Medical Research, Director General – Council of Scientific and Industrial Research, Director General – Health Services, Plant Protection Adviser, Directorate of Plant Protection, Quarantine and storage, Chairman, Central Pollution Control Board and three outside experts in individual capacity.
(iv) Member Secretary: An official of the Department of Environment, Forest and Wildlife.
The Committee may co-opt other members/experts as necessary.
The committee or any person/s authorised by it shall have powers to take actions under the Environment (Protection) Act.
(5) State Biotechnology Co-ordination Committee (SBCC).
There shall be a State Biotechnology Coordination Committee in the States wherever necessary. It shall have powers to inspect, investigate and take punitive action in case of violations of statutory provisions through the Nodal Department and the State Pollution Control board/Directorate of Health/Medical Services. The Committee shall review periodically the safety and control measures in the various industries/institutions handling genetically engineered Organisms/Hazardous microorganisms. The compositions of the Coordination Committee shall be:
(i) Chief Secretary – Chairman
(ii) Secretary, Department of Environment – Member Secretary
(iii) Secretary, Department of Health – Member
(iv) Secretary, Department of Agriculture – Member
(v) Secretary, Department of Industries and Commerce – Member
(vi) Secretary, Department of Forests – Member
(vii) Secretary, Department of Public Works/Chief Engineer,
Department of Public Health Engineering.- Member
(viii) State Microbiologists and Pathologists – Member
(ix) Chairman of State Pollution Control Board
The Committee may co-opt other members/experts as necessary.
(6) District Level Committee (DLC)
There shall be a District Level Biotechnology Committee (DLC) in the districts wherever necessary
under the District Collectors to monitor the safety regulations in installations engaged in the use of genetically modified organisms/hazardous microorganisms and its applications in the environment.
The District Level Committee/or any other person/s authorised in this behalf shall visit the installation engaged in activity involving genetically engineered organisms, hazardous microorganisms, formulate information chart, find out hazards and risks associated with each of these installations and coordinate activities with a view to meeting any emergency. They shall also prepare an off-site emergency plan. The District Level Committee shall regularly submit its report to the State Biotechnology Co-ordination Committee/Genetic Engineering Approval Committee.
The District Level Committee shall comprise of:-
(i) District Collector -Chairman
(ii) Factory Inspector -Member
(iii) A representative of the Pollution Control Board -Member
(iv) Chief Medical Officer (District Health Officer) -Member (Convenor)
(v) District Agricultural Officer -Member
(vi) A representative of the Public Health Engineering Department -Member
(vii) District Microbiologists/Pathologist (technical expert) – Member
(viii) Commissioner Municipal Corporation – Member
The committee may co-opt other members/experts as necessary
5. Classification of micro-organisms or genetically engineered product. – (1) For the purpose of these rules, microorganisms or genetically engineered organisms, products or cells shall be dealt with under two major heads; animal, pathogens and plant pests and these shall be classified in the manner specified in the Schedule.
(2)If any of the microorganisms, genetically engineered organism or cell falls within the limits of more than one risk class as specified in the Schedule, it shall be deemed to belong exclusively to the last in number of such classes.
6. Microorganisms laid down in the Schedule are divided into the following _
(i) Bacterial Agents;
(ii) Fungal Agents;
(iii) Parasitic Agents;
(iv) Viral, Rickettsial and Chlamydial Agents;
(v) Special Category.
7. Approval and Prohibitions, etc. – (1) No person shall import, export, transport, manufacture, process, use or sell any hazardous microorganisms of genetically engineered organisms/substances or cells except with the approval of the Genetic Engineering Approval Committee
Use of pathogenic microorgnisms or any genetically engineered organisms or cells for the purpose of research shall only be allowed in laboratories or inside laboratory area notified by the Ministry of Environment and Forests for this purpose under the Environment (Protection) Act, 1986.
(3) The Genetic Engineering Approval Committee shall give directions to the occupier to determine or take measures concerning the discharge of microorganisms/genetically engineered organisms or cells mentioned in the Schedule from the laboratories, hospitals and other areas including prohibition of such discharges and laying down measures to be taken to prevent such discharges.
(4) Any person operating or using genetically engineered organisms/microogranisms mentioned in the schedule for scale up or pilot operations shall have to obtain licence issued by the Genetic Engineering Approval Committee for any such activity. The possessor shall have to apply for licence in prescribed proform.
(5) Certain experiments for the purpose of education within the field of gene technology or microorganism may be carried out outside the laboratories and laboratory areas mentioned in sub-rule (2) and will be looked after by the Institutional Bio-safety Committee.
8. Production. – Production in which genetically engineered organisms or cells or micro-organisms are generated or used shall not be commenced except with the consent of Genetic Engineering Approval Committee with respect of discharge of genetically engineered organisms or cells into the environment. This shall also apply to production taking place in connection with development, testing and experiments where such production, etc., is not subject to rule 7.
9. Deliberate or unintentional release. – (1) Deliberate or unintentional release of genetically engineered organisms/hazardous microorganisms or cells, including deliberate release for the purpose of experiment shall not be allowed.
Note: Deliberate release shall mean any intentional transfer of genetically engineered organisms/hazardous, microorganisms or cells to the environment or nature, irrespective of the way in which it is done.
(2) The Genetic Engineering Approval Committee may in special cases give approval of deliberate release.
10. Permission and approval for certain substances. – Substances and products, which contain genetically engineered organisms or cells or microorganisms shall not be produced, sold, imported or used except with the approval of Genetic Engineering Approval Committee.
11. Permission and approval for food stuffs. – Food stuffs, ingredients in food stuffs and additives including processing and containing or consisting of genetically engineered organisms or cells, shall not be produced, sold, imported or used except with the approval of the Genetic Engineering Approval Committee.
12. Guidelines – (1) Any person who applies for approval under rules 8-11 shall, as determined by the Genetic Engineering Approval Committee submit information and make examinations or cause examinations to be made to eradicate the case, including examinations according to specific directions and at specific laboratories. He shall also make available an on-site emergency plan to GEAC before obtaining the approval. If the authority makes examination itself, it may order the applicant to delay the expenses incurred by it in so doing.
Any person to whom an approval has been granted under rules 8-11 above shall notify the Genetic Engineering Approval Committee of any change in or addition to the information already submitted.
13. Grant of approval. – (1) In connection with the granting of approval under rules 8 to 11 above, terms and conditions shall be stipulated, including terms and conditions as to the control to be exercised by the applicant, supervision, restriction on use, the layout of the enterprise and as to the submission of information to the State Bio-technology Co-ordination Committee or to the District Level Committee.
(3) All approvals of the Genetic Engineering Approval Committee shall be for a specific period not exceeding four year at the first instance renewable for 2 years at a time. The Genetic Engineering Approval Committee shall have powers to revoke such approval in the following situations:-
(a) If there is any new information as to the harmful effects of the genetically engineered organisms or cells.
(b) If the genetically engineered organisms or cells cause such damage to the environment, nature or health as could not be envisaged when the approval was given, or
(c) Non compliance of any condition stipulated by Genetic Engineering Approval Committee.
14. Supervision – (1) The Genetic Engineering Approval Committee may supervise the implementation of the terms and conditions laid down in connection with the approvals accorded by it.
The Genetic Engineering Approval Committee may carry out this supervision through the State Bio-technology Coordination Committee or the State Pollution Control Boards/District Level Committee or through any person authorised in this behalf.
15. Penalties. – (1) If an order is not complied with, the District Level Committee or State Biotechnology Co-ordination Committee may take measures at the expense of the person who is responsible.
In case where immediate intervention is required in order to prevent any damage to the environment, nature or health, the District Level Committee, or State Biotechnology Co-ordination Committee may take the necessary steps without issuing any order or notice. The expenses incurred for this purpose will be repayable by the person responsible for such damage.
The State Bio-technology Co-ordination Committee/District Level Committee may take samples for a more detailed examination of organisms and cells.
The State Bio-technology Co-ordination Committee/District Level Committee shall be competent to ask for assistance from any other government authority to carry out its instructions.
16. Responsibility to notify interruptions or accidents. – (1) Any person who under rule 7-11 is responsible for conditions or arrangements shall immediately notify the District Level Committee/State Biotechnology Co-ordination Committee and the state medical officer of any interruption of operations or accidents that may lead to discharges of genetically engineered organisms or cells which may be harmful to the environment, nature or health or involve any danger thereto.
(2) Any notice given under sub-rule (1) above shall not lessen the duty of the person who is responsible to try effectively to minimise or prevent the effects of interruptions of operations or accidents.
17. Preparation of Off-site emergency Plan by the DLC. – (1) It shall be the duty of the DLC to prepare an off-site emergency plan detailing how emergencies relating to a possible major accident at a site will be dealt with and in preparing the plan, the DLC shall consult the occupier and such other person as it may deem necessary.
(2) For the purpose of enabling the DLC to prepare the emergency plan required under sub-rule (1), the occupier shall provide the DLC with such information relating to the handling of hazardous microorganisms/genetically engineered organisms under his control as the DLC may required including the nature, extent and likely off-site effects of a possible major accident and the DLC shall provide the occupier with any information from the off-site emergency plan which relates to his duties under rule 16.
18. Inspections and informations regarding finance. – (1) The State Biotechnology Co-ordination Committee or the Genetic Engineering Approval Committee/the DLC or any person with special knowledge duly authorised by the State Bio-technology Co-ordination Committee or the Genetic Engineering Approval Committee or the DLC where it is deemed necessary, at any time on due production of identity be admitted to public as well as to private premises and localities for the purpose of carrying out supervision.
Any person who is responsible for activities subject to rules 7-11 above shall at the request of District Level Committee or State Bio-technology Co-ordination Committee or the GEAC submit all such information including information relating to financial conditions and accounts, as is essential to the authority’s administration under these rules. He shall also allow supervision or inspection by the authorities or persons indicated in sub-rule (1).
The Genetic Engineering Approval Committee may fix fees to cover, in whole or in part, the expenses incurred by the authorities in connection with approvals, examinations, supervisions and control.
19. Appeal – (1) Any person aggrieved by a decision made by Genetic Engineering Approval Committee/State Biotechnology Co-ordination Committee in pursuance of these rules may within thirty days from the date on which the decision is communicated to him, prefer an appeal to such authority as may be appointed by Ministry of Environment and Forests provided that the appellate authority may entertain the appeal after the expiry of the said period of thirty days if such authority is satisfied that the appellant was prevented by sufficient cause from filing the appeal in time.
20. Exemption – The Ministry of Environment and Forests shall, wherever necessary, exempt an occupier handling a particular microorganism genetically engineered organism from rule 7-11.
ANIMAL AND HUMAN PATHOGENS
SCHEDULE
BACTERIAL
Risk Group II
Acinetobacter calcoaceticus
Actinobacillus – all species except A -mallei, which in Risk Group III
Acromonoas hydrophila
Arzona hinshawii – all serotypes
Baciluss anthracis
Bordetella all species
Borrelia recurrentis B. vincenti
Campylobacter fetus
Camphylobacter jejuni
Chalamydia psittaci
Cheamydia trachomatis
Clostridium hauvoci, Cl, Difficle Cl. fallax, Cl haemolyticum Cl histolyticum, Cl, novyi, (Cl.perfringes), Cl. speticum, Cl. sordeili
Cornylebacterium diptheriae, C. equi, C. haemolyticum, C. pseudo tuberculosis, C. pyogenes,
C. renale
Diplococcus (Streptococcus) pneumoniae
Edwardsiila tarda
Erysipelothix insidiosa
Escherichia Coli-all enteropathogenic serotypes enterotoxigenic
Haemophilus ducrevi, H. influenzae, H. pneumoniae
Herellea vaginicola
Klebsiella-all species and all serotypes
Legionlla pneumophila
Letionella
Leptospira interrogans all serotypes reported in India
Listeria, all species
Mima polymorpha
Moraxella – All species
Mycobacteria – all species including Mycobacterium avium
M. bovis M. tuberculosis, M. lepreae
Mycoplasma – all species except M. mycoides and M. angalaectiae
Neisseric gonorrhoea, N. meningitis
Pasteurella – all species except those listed in Risk Group III
Salmonella – all species and all serotypes
Shigella – all species and all serotypes
Shpacrophorus necrophorus
Staphylococcus aureus
Streptobacillus moniformis
Streptococcus pneumoniae
Streptococcus pyogenes, S. equi
Streptomyces madurae, s. pelleteri, s. somaliensis
Treponema carateum, T. palidum and T. pertenue
Vibrio foctus, V. comma including biotype EI Tor and
V. parahemolyticus.
Vibrio cholerae
Risk Group III:
Actinobacillus mallei
Bartonella – all species
Brucella – all species
Clostridium botulium, Cl. tetani
Francisella tularensis
Mycobacterium avium, M. bovis, M. tuberculosis, m. leprae
Paseturella multocida type B (“buffalo” and other foreign virulent strains)
Pseudomonas pseudomallai
Yersinia pestis
FUNGAL
Risk Group II
Actinomycetes (including Nocardia Sp, Actinomyces species and Arachina prpinica)
Aspergilus fumigatus
Blastomyces dermatitis
Cryptococcus neofonnans C. fersiminosos
Epidermophyton madurella, microsporon
Paracoccidiodes brasiliensis
Sporothrix
Trichoderma
Trichophyton
Risk Group III:
Coccidiodes immitis Histoplasma capulatum
Histoplasma capsulatum var duboissl
PARASITIC
Risk Group II
Entahoeba histolytica
Leishmania species
Naegeleria gruberia
Plasmodium theilera, P. babesia P. falcoparum
Plasmodium babesia
Schistosoma
Toxoplasma gondii
Toxocara canis
Trichinella spiralis
Trichomanas
Trypanosoma cruzi
Risk Group III
Schisistosoma mansoni
VIRAL RICKETTSIAL AND CHAIMYDIAL
Risk Group II
Adenoviruses – Human, all types
Avian loukosis
Cache Valley virus
CELO (avain adenovirus)
Cosackie A and B viruses
Corona viruses
Cytomegalo viruses
Dengue virus, when used for transmission experiments
Echo viruses – all types
Encephalomyocarditis virus (EMC)
Flanders virus
Hart Part virus
Hepatitis-associated antigen material – hepatitis A and B viruses, non A and non B, HDV
Herpes viruses – except herpesvirus simiae (monkey B virus) which is in Risk Group IV.
Infections Bovine Rhinotraechitis virus (IBR)
Infections Bursal diesaes of poultry and Infectious Bronchitus
Infections Laryngotraechitis (ILT)
Influenza virus – all types, except A/PR8/34 which in Risk Group I
Langat virus Leucosis Complex
Lymphogranuloma venereum agent
Mark’s Disease virus
Measles virus
Mumps virus
Newcastle disease virus (other than licenced strain for vaccine use)
Parainfluenza viruses – all types except parainfluenza virus 3, SF4 strain, which is in Risk Group I
Polio viruses – all types wild and attenuated
Pox viruses – all types except Mathrim, monkey pox, sheep pox and white pox, which depending on experiments are in Risk Group III or IV.
Rabies virus – all strains except rabies stret virus, which should be classified in Risk Group
III when inoculated into comivores
Reoviruses – all types
Respiratory Syncital virus
Rhinoviruses – all types
Rinderpest (other than vaccine strain in use)
Rubella virus
Stimian viruses – all types except herpeavirus simae (Monkey Virus) which is in Risk Group IV
Simian virus 40 –
Ad SV 40 (defective)
Sindbis virus
Tensaw virus
Turlock virus
Vaccinia virus
Varicella virus
Vole rickettsia
Yellow fever virus, 17 Devaccinc strain
Risk Group III
African House Sickness (attenuated strain except animal passage)
Alastrim, monkey pox and whitepox, when used into vitro
Arboviruses – All strains exept those in Risk Group II and IV
Blue tongue virus (only serotypes reported in India)
Ebola fever Virus
Epstiein-Barr virus
Feline Leukemia
Feline sarcoma
Foot and Mouth Disease virus (all seritypes and subtypes)
Gibbon Ape Lymphosarcoma
herpesvirus ateles
herpevirus saimiri
herpes simplex 2
HIV-1 & HIV-2 and strains of SIV
Infectious Equine Anaemia
Lymphocytic choriomeningitis virus (LCM)
Monkey pox, when used in vitro
Nen-defective Adeno-2 SV-40 hybrids
Psettacosis-ornithosis-trachoma group of agents
Pseudorabies virus
Rabies street virus, when used inoculations of carnivores
Rickttsia – all species except Vole rickeitsia dn Coxiell burnetti when used for vector
transmission or animal inoculatin experiments
Sheep pox (field strain)
Swine Fever virus
Vesiculat stomatitis virus
Woolly monkey Fibrosarcoamm
Yaba pox virus
Risk Group IV
Alastrim, monkeypox, whitepox, when used for transimission or animal inoculation experiments
Hemorrhagic fever agents, including Crimean hemorrhagic fever (congo)
Korean hemorrhagic fever and others as yet undefined
Herpesvirus simlae (monkey B virus)
Tick-borne encephalitis virus complex, including – Russian Spring Summer Encephalitis,
Kyasanur Forest Diseast, omsk hemorrhagic fever and Central European encephalitis viruses.
Special Category
Bacterial
Contagious Equine Metritis (H. equinilais) Pestis petit de ruminantium
VIRAL RICKETTSIAL AND CHLAMYDIAL:
African Horse Sickness virus (serotypes not reported in Indian and challenge strains)
African Swine Fever
Bat rabies virus
Blue tongue virus (serotypes not reported in India)
Exoitic FMD virus types and sub-types
Junin and Machupo viruses
Lassa virus
Marburg virus
Murrey valley encephalitis virus
Rift Valley Fever virus
Smallpox virus-Archieval storage and propagation Swine Vesicular Disease
Veneseulan equine encephalitis virus epidemic strains
Western Equine encephalitis virus
Yellow fever virus – Wild strain
Other Arboviruses causing eizootics and so far not recorded in India.
B: PLANT PESTS
Any living stage (including active and dormant forms) of insects, mites, nematodes, slugs, snails, bacteria, fungi, protozoa, other parasitic plants or reproductive parts there of: viruses or any organisms similar to or allied with any of the foregoing; or any infectious agents or substances, which can directly or indirectly injure or cause disease or damage in or to any plants or parts thereof, or any processed, manufactured, or other products of plants are considered plant pests.
Organisms belonging to all lower Taxa contained within the group listed are also included.
1. Viruses-
All viruses
All bacteria, fungal, algal, plant, insect and neumatode viruses; special care should be taken for-
(i) Geminiviruses,
(ii) Calulimoviruses,
(iii) Nuclear Polyhedrosis viruses,
(iv) Grandulosis viruses, and
(v) Cytoplasmic polyhedrosis viruses
2 Bacteria
Family Pseudomonadaceae
Genus Pseudomonas
Genus Xanthomonas
Genus Azotobacter
Family Rhizobiaceae
Genus Rhizobium/Azorhizobium
Genus Bradyrhizobium
Genus Agrobacterium
Genus Phyllobacterium
Genus Erwinia
Genus Enterobacter
Genus Klebzieller
Family Spirollaceae
Genus Azospirillum
Genus Acqaspirillum
Genus Occeonospirillum
Family Streptomycetaceae
Genus Streptomyces
Genus Nocardia
Family Actiomycetaceas
Genus Actinomyces
Coryneform Group
Genus Clavibacter
Genus Arthrobacter
Genus Curtobacterium
Genus Bdellovibro
Family Rickettsiacea
Rickettsial like organisms associated with insect diseases
Gram-negative phloem-limited bacteria associated with plant diseases
Gram-negative xylem-limited bacteria associated with plant diseases
Cynobacteria – all members of blue-green algae
Mollicutes
Family Spiroplasmataceae
Mycoplasma-like organisms associated with plant diseases
Mycoplasma-like organisms associated with insect diseases
Algae
Family Chlorophyceae
Family Euglenophyceae
Family Pyrophyceae
Family Chrysophaceao
Family Phaephyceae
Family Rhodophyceae
Fungi
Family Plasmodiophoraceae
Family Chytridiaceae
Family Oldipiopsidaceae
Family Synchytriaceae
Family Catenariaceae
Family Coclomomycetaceae
Family Saprolegniaceae
Family Zoopagaceae
Family Albuginaceae
Family Peronosporaceae
Family Pythiaceae
Family Mucoraceae
Family Choanephoraceae
Family Mortierellaceae
Family Endogonaceae
Family Syncephalastraceae
Family Dimargaritaceae
Family Kiclxellaceae
Family Saksenaeaceae
Family Entomophthoraceae
Family Ecerinaceae
Family Taphrinaceae
Family Endomycetaceae
Family Sacharamycetacea
Family Eutotiaceae
Family Gymnoascaceae
Family Aseophaeriaceae
Family Onygenaceae
Family Microaseaceae
Family Protomycetaceae
Family Elsinocaceae
Family Myriaginaceae
Family Dothidiaceae
Family Chaetothyriaceae
Family Pharmulariaceae
Family Phillipsicaceae
Family Gysteriaceae
Family Pleosporaceae
Family Melamomataceae
Family Ophiostomataceae
Family Aseosphaeriaceae
Family Erysiphaceae
Family Meliolaceae
Family Xylariaceae
Family Diaporthaceae
Family Hypoercaceae
Family Clavicipataceae
Family Phacidiaceae
Family Ascocorticiaceae
Family Hemiphacidiaceae
Family Dermataceae
Family Selerotimiaceae
Family Cyttariaceae
Family Helosiaceae
Family Sarocostomataceae
Family Sarcoscyphaceae
Family Auricolariaceae
Family Ceratobasidiaceae
Family Corticiaceae
Family Hymenochaetaceae
Family Echiondintiaceae
Family Eistuliniaceae
Family Clavariaceae
Family Polyporaceae
Family Tricholomataceae
Family Ustilaginaceae
Family Sporobolomycetaceae
Family Uredinaceae
Family Agaricaceae
Family Graphiolaceae
Family Pucciniaceae
Family Melampsoraceae
Family Gandodermataceae
Family Labonlbeniaccue
Family Sphacropsidaceae
Family Melabconiaceae
Family Tuberculariaceae
Family Dematiaceae
Family Moniliaceae
Family Aganomucetaceae
Parasitic Weeds
Family Balanophoraceae-parasitic species
Family Cuscutaceae-parasitic species
Family Thydonoraceae-parasitic species
Family Lauraceae-parasitic species Genus Cassytha
Family Lennoaceae-parasitic species
Family Loranthaceae-parasitic species
Family Myzodendraceas-parasitic species
Family Olacaceae-parasitic species
Family Orobanchaceae-parasitic species
Family Rafflesiaceae-parasitic species
Family Santalaceae-parasitic species
Family Scrophulariaceae-parasitic species
Protozoa
Genus Phytomonas
And all Protozoa associated with insect dest
Nematodes
Family Anguinidae
Family Belonolaimidae
Family caloosiidae
Family Cariconematidae
Family dolichodoridae
Family Fergusbbiidae
Family hemicycliophoridae
Family Heteroderidae
Family Hoplolaimidae
Family Meloidogynidae
Family Neotylenchidae
Family Nothotylenchaidae
Family Paratylenchidae
Family Pratylenchidae
Family Tylenchidae
Family Tylenchulidae
Family Aphelenchoidae
Family Longdoridae
Family Trichodoridae
Mollusca
Super family Planorbacea
Super family Achatinacea
Super family Arionacea
Super family Limacacea
Super family Helicacea
Super family Veronicellacea
Arthropoda
Super family Ascoidea
Super family Dermanyssoidea
Super family Erjophyoidea
Super family Tetranychoidea
Super family Tetranychoidea
Super family Eupododea
Super family Tydeoidea
Super family Erythraenoidea
Super family Trombidioidea
Super family Hydryphantoidea
Super family Tarasonemoidea
Super family Pycmotoiodea
Super family Hemisaracoptoidea
Super family Acaroidea
Order Polydesmida
Family Sminthoridao
Family Forfieulidzo
Order Isptera
Order Thysanoptera
Family Acredidea
Family Gryllidae
Family Gryllacridiedae
Family Gryllotalpidae
Family Phasmatidao
Family Ronalcidao
Family Tettigoniidao
Family Tatragidao
Family Thaumastocoridae
Superfamily Piesmatoidea
Super family Lygacoidea
Super family Idiostoloidea
Super family Carcoidea
Super family Penatomoidea
Super family Pyrrhocomidea
Super family Tingoidea
Super family Miroidea
Order Homoptara
Family Anobiidae
Family Apionidae
Family Anthrididae
Family Bostrichidae
Family Brentidae
Family Bruchidae
Family Buprestodae
Family Byturidae
Family Cantharidae
Family Carabidae
Family Ceambiceidae
Family Chrysomelidae
Family Coecinellidae
Family Curculionidae
Family Dermestidae
Family Elateridae
Family Hydrophilidae
Family Lyctidae
Family Meloidae
Family Moredellidae
Family Platypodiadae
Family Scarabaeldae
Family Scolytidae
Family Selbytidae
Order Lepidoptera
Family Agromyidae
Family Anthomiidae
Family Cecidomiidae
Family Chioropidae
Family Ephydridae
Family Lonchacidae
Family Musicdae
Family Otitidae
Family Syrphidae
Family Tephritidae
Family Tipulidae
Family Apidae
Family Caphidae
Family Chalcidae
Family Cynipidae
Family Eurytomidae
Family formicidae
Family Psilidae
Family Sircidae
Family Tenthredinidae
Family Torymidae
Family Xyloiopidae
and
also unclassified organism and/or organisms whose classification is unknown, and all other
organisms associated with plant and insect disease.
————————————————————
Published in the Gazette No. 621 dt. 5-12-89
Corrections made in terms of corrigendum No. G.S.R. 137(E) dt. 21-2-90 published in the Gazette.
INISTRY OF ENVIRONMENT AND FORESTSNOTIFICATION
New Delhi, the 9th July, 2002
Environment (Protection) Third Amendment Rules, 2002.
G.S.R- 489(E).–In exercise of the powers conferred by sections 6 and 25 of the Environment (Protection).Act, 1986 (29 of 1986), the Central Government hereby makes the following rules further to amend the Environment (Protection) Rules, 1986, namely:-
1. (1) These rules may be called the Environment (Protection) Third Amendment Rules, 2002.
(2) They shall come into force on the date of their publication in the Official Gazette.
2. In the Environment (Protection) Rules, 1986, in Schedule 1, after serial number 95 relating to Emission Limits for New Diesel Engines (up to 800 KW) for Generator Sets (Gensets) Applications and the entries relating thereto, the following serial number and entries shall be inserted, namely :
“96. EMISSION STANDARDS FOR DIESEL ENGINES (ENGINE : RATING MORE THAN 0.8 MW (80.0 KW) FOR POWER PLANT, GENERATOR SET APPLICATIONS AND OTHER REQUIREMENTS
TABLE
Parameter Area Category Total engine rating of the plant (includes existing as well as new generators sets)t Generator sets commissioning date
Before 1.7.2003 Between 1.7.2003 and 1.7.2005 On or after 1.7.2005
NOX (as NO2)
(AT 15% O2), dry basis, in
ppmv
A Upto 75 MW 1100 970 710
B Upto 50 MW
A More than 75 MW 1100 710 360
B More than 150 MW
NMHC (as C) (at 15% O2), mg/Nm3 Both A and B 150 100
PM (at 15% O2), mg/NM3. Diesel
Fuels –
HSD & LDO
Both A and B 75 75
Furnace Oils-LSHS & FO Both A and B 150 100
CO (at 15% O2, mg/Nm3 Both A and B 150 150
Sulphur content in fuel A <2%
B <4%
Fuel specification For A only Up to 5MW Only Diesel Fuels (HSD, LDO) shall be used.
Stack height (for generator sets commissioned after 1.7.2003) Stack height shall be maximum of the following, in meter :
(i) 14 Q0.3., Q = Total SO2 emission from the plant in kg/hr.
(ii) Minimum 6 m. above the building where generator set is installed.
(iii) 30 m.
Â
MW : Mega (106) Watt
NOx : Oxides of Nitrogen
NO2 : Nitrogen Dioxide
O2 : Oxygen
NMHC : Non-Methane Hydrocarbon
C : Carbon
PM : Particulate Matter
CO : Carbon Monoxide
SO2 : Sulphur Dioxide
ppmv : Part per million (106) by volume
FO : Furnace Oil
HSD : High Speed Diesel
LDO : Light Diesel Oil
LSHS : Low Sulphur Heavy Stock
kPa : Kilo Pascal
mm : Milli (10-3) metre
kg/hr : Kilo (103) gram per hour
mg/Nm3 : Milli (10-3) gram per Normal metre cubic
2. Area categories A and B are defined as follows :
Category A: Areas within the municipal limits of towns/cities having population more than 10 lakhs and also upto 5 km beyond the municipal limits of such towns/cities.
Category B: Areas not covered by category A.
3. The standards shall be regulated by the State Pollution Control Boards or Pollution Control Committees, as the case may be.
4. Individual units with engine ratings less than or equal to 800 KW are not covered by this notification.
5. Only following liquid fuels viz. High Speed Diesel, Light Diesel oil, Low Sulphur Heavy Stock and Furnace Oil or liquid fuels with equivalent specifications shall be used in these power plants and generator sets.
6. For expansion project, stack height of new generator sets shall be as per total Sulphur Dioxide emission (including existing as well as additional load).
7. For multi engine plants, fuels shall be grouped in cluster to get better plume rise and dispersion. Provision for any future expansion should be made in planning stage itself.
8. Particulate Matter, Non-Methane Hydrocarbon and Carbon Monoxide results are to be normalized to 25? C, 1.01 Kilo Pascal (760 mm of mercury) pressure and zero percent moisture (dry basis).
9. Measurement shall be performed at steady load conditions of more than 85% of the rated load.
10. Continuous monitoring of Oxides of Nitrogen shall be done by the plants whose total engine capacity is more than 50 Mega Watt. However, minimum once in six month monitoring for other parameters shall be adopted by the plants.
11. Following methods may be adopted for the measurement of emission parameters,-
Sl. No. Emission Parameters Measurement Methods
1. Particulates Gravimetric
2. SO2 Barium Perchlorate-Thorin indicator method
3. NOx Chemiluminescence, Non Dispersive Infra Red, Non Dispersive Ultra-violet (for continuous measurement), Phenol disulphonic method
4. CO Non Dispersive Infra Red
5. O2 Paramagnetic, Electrochemical sensor
6. NHMC Gas Chromatograph – Flame Ionisation Detector
[F. No. Q-15017/24/2002-CPW]
C. VISWANATH, Jt. Secy.
Note :- The principal rules were published in the Gazette. of India vide number S.O. 844(E) 19th November, 1986 and subsequently amended vide S.O. 433(E) dated 18th April, 1987, S.O. 64(E) dated 18th January, 1988, S.0.3(E) dated 3rd January. 1989. S.O. 190(E) dated 15th March, 1989, G.S.R. 913(E) the 24th October, 1989, S.O. 12(E) dated the 8th January, 1990, G.S.R. 742(E) dated the 30th August, 1990, S.O. 23(E) dated the 16th January, 1991, G.S.R. 93(E) dated the 21st February, 1991, G.S.R. 95(E) dated the 12th February, 1992, G.S.R. 329(E) dated the 13th March, 1992, G.S.R. 475(E) dated the 5th May, 1992, G.S.R. 797(E) dated the 1st October, 1992, G.S.R. 386(E) dated the 28th April, 1993, G.S.R. 422(E) dated the 19th May, 1993, G.S.R. 801(E) dated the 31st December, 1993, G.S.R. 176(E) dated the 3rd April 1996, G.S.R. 631(E) dated the 31st October, 1997, G.S.R. 504(E) dated the 20th August, 1998, G.S.R. 7(E) dated the 25th September, 2000, G.S.R. 72(E) dated 6th February, 2001, G.S.R. 54(E) dated 22.1.2002 and G.S.R. No. 371(E) dated 17.5.2002.
